Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria

Native cell-free transcription–translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host, Bacillus megate...

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Bibliographic Details
Main Authors: Moore, S, Macdonald, J, Wienecke, S, Ishwarbhai, A, Tsipa, A, Aw, R, Kylilis, N, Bell, D, McClymont, D, Jensen, K, Polizzi, K, Biedendieck, R, Freemont, P
Format: Journal article
Language:English
Published: National Academy of Sciences 2018