Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression.
Interleukin 10 is a potent anti-inflammatory and immunomodulatory cytokine. Little is known regarding its induction in monocytes/macrophages, however LPS, a reproducible trigger of IL-10, is augmented by direct contact with T cells. In this context, the role of CD40-ligation is investigated. In the...
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1826271385202196480 |
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| author | Foey, A Feldmann, M Brennan, F |
| author_facet | Foey, A Feldmann, M Brennan, F |
| author_sort | Foey, A |
| collection | OXFORD |
| description | Interleukin 10 is a potent anti-inflammatory and immunomodulatory cytokine. Little is known regarding its induction in monocytes/macrophages, however LPS, a reproducible trigger of IL-10, is augmented by direct contact with T cells. In this context, the role of CD40-ligation is investigated. In the rheumatoid synovium, IL-10 is produced by tissue macrophages. Monocytes primed with M-CSF, a cytokine present in rheumatoid joints, produced IL-1beta, TNF-alpha and IL-10 upon CD40-ligation at an IL-1: TNF-alpha: IL-10 ratio of 10:0.5:1. IFN-gamma-primed monocytes, however, predominantly produced TNF-alpha and IL-1beta. Both differentiated monocytes display an endogenous IL-10 activity regulatable by CD40 stimulation. Additionally, these monocytes display differential control by exogenous and endogenous IL-1 and TNF-alpha. M-CSF-primed monocyte IL-10 production was dependent on endogenous TNF-alpha and, to a lesser extent, IL-1, whereas IFN-gamma-primed monocytes were partially dependent on endogenous IL-1. The addition of exogenous IL-1 augments CD40 induced IL-10 production by IFN-gamma-primed monocytes. These data indicate that CD40 ligation regulates cell contact mediated macrophage IL-10 and that the route of differentiation determines the cytokine profile. |
| first_indexed | 2024-03-06T21:55:50Z |
| format | Journal article |
| id | oxford-uuid:4ce75f83-bc7e-4bf0-a792-89eb3ece6fa9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:55:50Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:4ce75f83-bc7e-4bf0-a792-89eb3ece6fa92022-03-26T15:52:08ZRoute of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4ce75f83-bc7e-4bf0-a792-89eb3ece6fa9EnglishSymplectic Elements at Oxford2000Foey, AFeldmann, MBrennan, FInterleukin 10 is a potent anti-inflammatory and immunomodulatory cytokine. Little is known regarding its induction in monocytes/macrophages, however LPS, a reproducible trigger of IL-10, is augmented by direct contact with T cells. In this context, the role of CD40-ligation is investigated. In the rheumatoid synovium, IL-10 is produced by tissue macrophages. Monocytes primed with M-CSF, a cytokine present in rheumatoid joints, produced IL-1beta, TNF-alpha and IL-10 upon CD40-ligation at an IL-1: TNF-alpha: IL-10 ratio of 10:0.5:1. IFN-gamma-primed monocytes, however, predominantly produced TNF-alpha and IL-1beta. Both differentiated monocytes display an endogenous IL-10 activity regulatable by CD40 stimulation. Additionally, these monocytes display differential control by exogenous and endogenous IL-1 and TNF-alpha. M-CSF-primed monocyte IL-10 production was dependent on endogenous TNF-alpha and, to a lesser extent, IL-1, whereas IFN-gamma-primed monocytes were partially dependent on endogenous IL-1. The addition of exogenous IL-1 augments CD40 induced IL-10 production by IFN-gamma-primed monocytes. These data indicate that CD40 ligation regulates cell contact mediated macrophage IL-10 and that the route of differentiation determines the cytokine profile. |
| spellingShingle | Foey, A Feldmann, M Brennan, F Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title | Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title_full | Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title_fullStr | Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title_full_unstemmed | Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title_short | Route of monocyte differentiation determines their cytokine production profile: CD40 ligation induces interleukin 10 expression. |
| title_sort | route of monocyte differentiation determines their cytokine production profile cd40 ligation induces interleukin 10 expression |
| work_keys_str_mv | AT foeya routeofmonocytedifferentiationdeterminestheircytokineproductionprofilecd40ligationinducesinterleukin10expression AT feldmannm routeofmonocytedifferentiationdeterminestheircytokineproductionprofilecd40ligationinducesinterleukin10expression AT brennanf routeofmonocytedifferentiationdeterminestheircytokineproductionprofilecd40ligationinducesinterleukin10expression |