Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases.
The association of HLA-B27 with ankylosing spondylitis and reactive arthritis is the strongest one known between an MHC class I Ag and a disease. We have searched the proteome of the bacterium Chlamydia trachomatis for HLA-B27 binding peptides that are stimulatory for CD8(+) cells both in a model of...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2001
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| _version_ | 1826271521640808448 |
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| author | Kuon, W Holzhütter, H Appel, H Grolms, M Kollnberger, S Traeder, A Henklein, P Weiss, E Thiel, A Lauster, R Bowness, P Radbruch, A Kloetzel, P Sieper, J |
| author_facet | Kuon, W Holzhütter, H Appel, H Grolms, M Kollnberger, S Traeder, A Henklein, P Weiss, E Thiel, A Lauster, R Bowness, P Radbruch, A Kloetzel, P Sieper, J |
| author_sort | Kuon, W |
| collection | OXFORD |
| description | The association of HLA-B27 with ankylosing spondylitis and reactive arthritis is the strongest one known between an MHC class I Ag and a disease. We have searched the proteome of the bacterium Chlamydia trachomatis for HLA-B27 binding peptides that are stimulatory for CD8(+) cells both in a model of HLA-B27 transgenic mice and in patients. This was done by combining two biomathematical computer programs, the first of which predicts HLA-B27 peptide binding epitopes, and the second the probability of HLA-B27 peptide generation by the proteasome system. After preselection, immunodominant peptides were identified by Ag-specific flow cytometry. Using this approach we have identified for the first time nine peptides derived from different C. trachomatis proteins that are stimulatory for CD8(+) T cells. Eight of these nine murine-derived peptides were recognized by cytotoxic T cells. The same strategy was used to identify B27-restricted chlamydial peptides in three patients with reactive arthritis. Eleven peptides were found to be stimulatory for patient-derived CD8(+) T cells, of which eight overlapped those found in mice. Additionally, we applied the tetramer technology, showing that a B27/chlamydial peptide containing one of the chlamydial peptides stained CD8(+) T cells in patients with Chlamydia-induced arthritis. This comprehensive approach offers the possibility of clarifying the pathogenesis of B27-associated diseases. |
| first_indexed | 2024-03-06T21:57:58Z |
| format | Journal article |
| id | oxford-uuid:4d95db9f-7173-454d-978b-780f5778d6e6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:57:58Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:4d95db9f-7173-454d-978b-780f5778d6e62022-03-26T15:56:19ZIdentification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4d95db9f-7173-454d-978b-780f5778d6e6EnglishSymplectic Elements at Oxford2001Kuon, WHolzhütter, HAppel, HGrolms, MKollnberger, STraeder, AHenklein, PWeiss, EThiel, ALauster, RBowness, PRadbruch, AKloetzel, PSieper, JThe association of HLA-B27 with ankylosing spondylitis and reactive arthritis is the strongest one known between an MHC class I Ag and a disease. We have searched the proteome of the bacterium Chlamydia trachomatis for HLA-B27 binding peptides that are stimulatory for CD8(+) cells both in a model of HLA-B27 transgenic mice and in patients. This was done by combining two biomathematical computer programs, the first of which predicts HLA-B27 peptide binding epitopes, and the second the probability of HLA-B27 peptide generation by the proteasome system. After preselection, immunodominant peptides were identified by Ag-specific flow cytometry. Using this approach we have identified for the first time nine peptides derived from different C. trachomatis proteins that are stimulatory for CD8(+) T cells. Eight of these nine murine-derived peptides were recognized by cytotoxic T cells. The same strategy was used to identify B27-restricted chlamydial peptides in three patients with reactive arthritis. Eleven peptides were found to be stimulatory for patient-derived CD8(+) T cells, of which eight overlapped those found in mice. Additionally, we applied the tetramer technology, showing that a B27/chlamydial peptide containing one of the chlamydial peptides stained CD8(+) T cells in patients with Chlamydia-induced arthritis. This comprehensive approach offers the possibility of clarifying the pathogenesis of B27-associated diseases. |
| spellingShingle | Kuon, W Holzhütter, H Appel, H Grolms, M Kollnberger, S Traeder, A Henklein, P Weiss, E Thiel, A Lauster, R Bowness, P Radbruch, A Kloetzel, P Sieper, J Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title | Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title_full | Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title_fullStr | Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title_full_unstemmed | Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title_short | Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. |
| title_sort | identification of hla b27 restricted peptides from the chlamydia trachomatis proteome with possible relevance to hla b27 associated diseases |
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