Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficienci...

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Main Authors: Hellenkamp, B, Schmid, S, Doroshenko, O, Opanasyuk, O, Kühnemuth, R, Rezaei Adariani, S, Ambrose, B, Aznauryan, M, Barth, A, Birkedal, V, Bowen, M, Chen, H, Cordes, T, Eilert, T, Fijen, C, Gebhardt, C, Götz, M, Gouridis, G, Gratton, E, Ha, T, Hao, P, Hanke, C, Hartmann, A, Hendrix, J, Hildebrandt, L, Hirschfeld, V, Hohlbein, J, Hua, B, Hübner, C, Kallis, E, Kapanidis, A, Kim, J, Krainer, G, Lamb, D, Lee, N, Lemke, E, Levesque, B, Levitus, M, McCann, J, Naredi-Rainer, N, Nettels, D, Ngo, T, Qiu, R, Robb, N, Röcker, C, Sanabria, H, Schlierf, M, Schröder, T, Schuler, B, Seidel, H, Streit, L, Thurn, J, Tinnefeld, P, Tyagi, S, Vandenberk, N, Vera, A, Weninger, K, Wünsch, B, Yanez-Orozco, I, Michaelis, J, Seidel, C, Craggs, T, Hugel, T
Format: Journal article
Language:English
Published: Springer Nature Publishing Group 2018

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