Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficienci...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Springer Nature Publishing Group
2018
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| _version_ | 1826271679560548352 |
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| author | Hellenkamp, B Schmid, S Doroshenko, O Opanasyuk, O Kühnemuth, R Rezaei Adariani, S Ambrose, B Aznauryan, M Barth, A Birkedal, V Bowen, M Chen, H Cordes, T Eilert, T Fijen, C Gebhardt, C Götz, M Gouridis, G Gratton, E Ha, T Hao, P Hanke, C Hartmann, A Hendrix, J Hildebrandt, L Hirschfeld, V Hohlbein, J Hua, B Hübner, C Kallis, E Kapanidis, A Kim, J Krainer, G Lamb, D Lee, N Lemke, E Levesque, B Levitus, M McCann, J Naredi-Rainer, N Nettels, D Ngo, T Qiu, R Robb, N Röcker, C Sanabria, H Schlierf, M Schröder, T Schuler, B Seidel, H Streit, L Thurn, J Tinnefeld, P Tyagi, S Vandenberk, N Vera, A Weninger, K Wünsch, B Yanez-Orozco, I Michaelis, J Seidel, C Craggs, T Hugel, T |
| author_facet | Hellenkamp, B Schmid, S Doroshenko, O Opanasyuk, O Kühnemuth, R Rezaei Adariani, S Ambrose, B Aznauryan, M Barth, A Birkedal, V Bowen, M Chen, H Cordes, T Eilert, T Fijen, C Gebhardt, C Götz, M Gouridis, G Gratton, E Ha, T Hao, P Hanke, C Hartmann, A Hendrix, J Hildebrandt, L Hirschfeld, V Hohlbein, J Hua, B Hübner, C Kallis, E Kapanidis, A Kim, J Krainer, G Lamb, D Lee, N Lemke, E Levesque, B Levitus, M McCann, J Naredi-Rainer, N Nettels, D Ngo, T Qiu, R Robb, N Röcker, C Sanabria, H Schlierf, M Schröder, T Schuler, B Seidel, H Streit, L Thurn, J Tinnefeld, P Tyagi, S Vandenberk, N Vera, A Weninger, K Wünsch, B Yanez-Orozco, I Michaelis, J Seidel, C Craggs, T Hugel, T |
| author_sort | Hellenkamp, B |
| collection | OXFORD |
| description | Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between ±0.02 and ±0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and discuss potential uncertainties in the experiment and the modeling. Our quantitative assessment of the reproducibility of intensity-based smFRET measurements and a unified correction procedure represents an important step toward the validation of distance networks, with the ultimate aim of achieving reliable structural models of biomolecular systems by smFRET-based hybrid methods. |
| first_indexed | 2024-03-06T22:00:28Z |
| format | Journal article |
| id | oxford-uuid:4e671113-967a-4fbd-bb06-dfcf381cfd8e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:00:28Z |
| publishDate | 2018 |
| publisher | Springer Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:4e671113-967a-4fbd-bb06-dfcf381cfd8e2022-03-26T16:01:07ZPrecision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark studyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4e671113-967a-4fbd-bb06-dfcf381cfd8eEnglishSymplectic Elements at OxfordSpringer Nature Publishing Group2018Hellenkamp, BSchmid, SDoroshenko, OOpanasyuk, OKühnemuth, RRezaei Adariani, SAmbrose, BAznauryan, MBarth, ABirkedal, VBowen, MChen, HCordes, TEilert, TFijen, CGebhardt, CGötz, MGouridis, GGratton, EHa, THao, PHanke, CHartmann, AHendrix, JHildebrandt, LHirschfeld, VHohlbein, JHua, BHübner, CKallis, EKapanidis, AKim, JKrainer, GLamb, DLee, NLemke, ELevesque, BLevitus, MMcCann, JNaredi-Rainer, NNettels, DNgo, TQiu, RRobb, NRöcker, CSanabria, HSchlierf, MSchröder, TSchuler, BSeidel, HStreit, LThurn, JTinnefeld, PTyagi, SVandenberk, NVera, AWeninger, KWünsch, BYanez-Orozco, IMichaelis, JSeidel, CCraggs, THugel, TSingle-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between ±0.02 and ±0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and discuss potential uncertainties in the experiment and the modeling. Our quantitative assessment of the reproducibility of intensity-based smFRET measurements and a unified correction procedure represents an important step toward the validation of distance networks, with the ultimate aim of achieving reliable structural models of biomolecular systems by smFRET-based hybrid methods. |
| spellingShingle | Hellenkamp, B Schmid, S Doroshenko, O Opanasyuk, O Kühnemuth, R Rezaei Adariani, S Ambrose, B Aznauryan, M Barth, A Birkedal, V Bowen, M Chen, H Cordes, T Eilert, T Fijen, C Gebhardt, C Götz, M Gouridis, G Gratton, E Ha, T Hao, P Hanke, C Hartmann, A Hendrix, J Hildebrandt, L Hirschfeld, V Hohlbein, J Hua, B Hübner, C Kallis, E Kapanidis, A Kim, J Krainer, G Lamb, D Lee, N Lemke, E Levesque, B Levitus, M McCann, J Naredi-Rainer, N Nettels, D Ngo, T Qiu, R Robb, N Röcker, C Sanabria, H Schlierf, M Schröder, T Schuler, B Seidel, H Streit, L Thurn, J Tinnefeld, P Tyagi, S Vandenberk, N Vera, A Weninger, K Wünsch, B Yanez-Orozco, I Michaelis, J Seidel, C Craggs, T Hugel, T Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title | Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title_full | Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title_fullStr | Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title_full_unstemmed | Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title_short | Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study |
| title_sort | precision and accuracy of single molecule fret measurements a multi laboratory benchmark study |
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