DNA duplexes stabilized by modified monomer residues: synthesis and stability
The synthesis of a series of 2′-deoxyuridine analogues modified at the 5-position and a series of 2′-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications....
| Main Authors: | , , |
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| Format: | Journal article |
| Jezik: | English |
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1998
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| _version_ | 1826271680146702336 |
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| author | Graham, D Parkinson, J Brown, T |
| author_facet | Graham, D Parkinson, J Brown, T |
| author_sort | Graham, D |
| collection | OXFORD |
| description | The synthesis of a series of 2′-deoxyuridine analogues modified at the 5-position and a series of 2′-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications. Of those modified residues which have been incorporated into oligonucleotides, 5-(prop-1-ynyl)-2′-deoxyuridine is found to impart the greatest increase in stability on the DNA duplexes studied. Thermodynamic analyses show that the reason for this increase in stability arises from a decrease in enthalpy which is related to the DNA base stacking process. We suggest that the degree of stabilisation imparted by the propyne moiety may be sequence dependent. |
| first_indexed | 2024-03-06T22:00:29Z |
| format | Journal article |
| id | oxford-uuid:4e675b2c-247f-48d6-8ff9-fc130d52a80c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:00:29Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:4e675b2c-247f-48d6-8ff9-fc130d52a80c2022-03-26T16:01:01ZDNA duplexes stabilized by modified monomer residues: synthesis and stabilityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4e675b2c-247f-48d6-8ff9-fc130d52a80cEnglishSymplectic Elements at Oxford1998Graham, DParkinson, JBrown, TThe synthesis of a series of 2′-deoxyuridine analogues modified at the 5-position and a series of 2′-deoxypurines modified at the 8-position is described. Ultraviolet melting studies have been used to assess the stability of DNA duplexes 12- and 8-residues in length that contain these modifications. Of those modified residues which have been incorporated into oligonucleotides, 5-(prop-1-ynyl)-2′-deoxyuridine is found to impart the greatest increase in stability on the DNA duplexes studied. Thermodynamic analyses show that the reason for this increase in stability arises from a decrease in enthalpy which is related to the DNA base stacking process. We suggest that the degree of stabilisation imparted by the propyne moiety may be sequence dependent. |
| spellingShingle | Graham, D Parkinson, J Brown, T DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title | DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title_full | DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title_fullStr | DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title_full_unstemmed | DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title_short | DNA duplexes stabilized by modified monomer residues: synthesis and stability |
| title_sort | dna duplexes stabilized by modified monomer residues synthesis and stability |
| work_keys_str_mv | AT grahamd dnaduplexesstabilizedbymodifiedmonomerresiduessynthesisandstability AT parkinsonj dnaduplexesstabilizedbymodifiedmonomerresiduessynthesisandstability AT brownt dnaduplexesstabilizedbymodifiedmonomerresiduessynthesisandstability |