Use of the nested reverse transcription-polymerase chain reaction for the detection of human papillomavirus 16 E6 transcriptional activity in cervical cancer: a technical perspective.

AIM: The aim of this study was to evaluate HPV 16 E6 expression using nested RT-PCR in cervical tumour tissue and compare this technique with standard RT-PCR in a group of patients using injectable contraceptive steroids. PATIENTS AND METHODS: Tumour DNA was analysed for the presence and type of HPV...

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Bibliographic Details
Main Authors: Moodley, M, Moodley, J, Herrington, C, Sewart, S, Chetty, R
Format: Journal article
Language:English
Published: 2004