Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR
<p style="text-align:justify;"> <b> Background:</b> Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
BioMed Central
2009
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