Removal of detergents from protein extracts using activated charcoal prior to immunological analysis.
The use of dextran-coated activated charcoal (DCC) powder to absorb solubilising detergents from cell lysates is described. Normal embryonic epithelial cells were lysed in the presence of sodium dodecyl sulphate (SDS). The detergent was then absorbed with DCC to facilitate analysis of polycystin-1 w...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2002
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