Assembly of a biocompatible triazole-linked gene by one-pot click-DNA ligation

The chemical synthesis of oligonucleotides and their enzyme-mediated assembly into genes and genomes has significantly advanced multiple scientific disciplines. However, these approaches are not without their shortcomings; enzymatic amplification and ligation of oligonucleotides into genes and genom...

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Bibliographic Details
Main Authors: Kukwikila, M, Gale, N, El-Sagheer, A, Brown, T, Tavassoli, A
Format: Journal article
Published: Nature Publishing Group 2017