Assembly of a biocompatible triazole-linked gene by one-pot click-DNA ligation
The chemical synthesis of oligonucleotides and their enzyme-mediated assembly into genes and genomes has significantly advanced multiple scientific disciplines. However, these approaches are not without their shortcomings; enzymatic amplification and ligation of oligonucleotides into genes and genom...
Main Authors: | , , , , |
---|---|
Format: | Journal article |
Published: |
Nature Publishing Group
2017
|