The stability of triplex DNA is affected by the stability of the underlying duplex.
We have studied the formation of DNA triple helices in different sequence contexts and show that, for the most stable triplexes, their apparent stability is affected by the stability of the underlying duplex. For a 14-mer parallel triplex-forming oligonucleotide (generating C(+).GC and T.AT triplets...
| Main Authors: | , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2009
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| _version_ | 1826272704501645312 |
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| author | Rusling, D Rachwal, P Brown, T Fox, K |
| author_facet | Rusling, D Rachwal, P Brown, T Fox, K |
| author_sort | Rusling, D |
| collection | OXFORD |
| description | We have studied the formation of DNA triple helices in different sequence contexts and show that, for the most stable triplexes, their apparent stability is affected by the stability of the underlying duplex. For a 14-mer parallel triplex-forming oligonucleotide (generating C(+).GC and T.AT triplets) at pH 5.0 the T(m) is more than 10 degrees C lower with an intermolecular 14-mer duplex target, than it is with an intramolecular duplex, or one which is flanked by 6 GC base pairs at either end. A similar effect is seen with triplex-forming oligonucleotides that contain stabilising analogues, for which the T(m) is higher for an intramolecular than an intermolecular duplex target. These results suggest that the use of simple intermolecular duplex targets may underestimate the triplex stabilisation that is produced by some nucleotide analogues. |
| first_indexed | 2024-03-06T22:16:48Z |
| format | Journal article |
| id | oxford-uuid:53a4b368-6b80-4219-abe6-64b7315057e3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:16:48Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:53a4b368-6b80-4219-abe6-64b7315057e32022-03-26T16:32:57ZThe stability of triplex DNA is affected by the stability of the underlying duplex.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:53a4b368-6b80-4219-abe6-64b7315057e3EnglishSymplectic Elements at Oxford2009Rusling, DRachwal, PBrown, TFox, KWe have studied the formation of DNA triple helices in different sequence contexts and show that, for the most stable triplexes, their apparent stability is affected by the stability of the underlying duplex. For a 14-mer parallel triplex-forming oligonucleotide (generating C(+).GC and T.AT triplets) at pH 5.0 the T(m) is more than 10 degrees C lower with an intermolecular 14-mer duplex target, than it is with an intramolecular duplex, or one which is flanked by 6 GC base pairs at either end. A similar effect is seen with triplex-forming oligonucleotides that contain stabilising analogues, for which the T(m) is higher for an intramolecular than an intermolecular duplex target. These results suggest that the use of simple intermolecular duplex targets may underestimate the triplex stabilisation that is produced by some nucleotide analogues. |
| spellingShingle | Rusling, D Rachwal, P Brown, T Fox, K The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title | The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title_full | The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title_fullStr | The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title_full_unstemmed | The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title_short | The stability of triplex DNA is affected by the stability of the underlying duplex. |
| title_sort | stability of triplex dna is affected by the stability of the underlying duplex |
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