Synthetic chemical inducers and genetic decoupling enable orthogonal control of the rhaBAD promoter.

External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD promoter has properties superior to more commonly-used inducible expression systems, but i...

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Bibliographic Details
Main Authors: Wormald, M, Jenkinson, S, Fleet, G, Kelly, C, Liu, Z, Yoshihara, A, Otero, J, Estevez, A, Kato, A, Marqvorsen, M, Estevez, R, Izumori, K, Heap, J
Format: Journal article
Published: American Chemical Society 2016