New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes.
The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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