Time-resolved fluorometry in end-point and real-time PCR quantification of nucleic acids.
Two time-resolved fluorescence-based methods for nucleic acid quantification are described and their results are compared. Both methods use an exogenous internal standard to eliminate errors arising from different steps of the assay. The first method is a competitive end-point assay, where the stand...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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