Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy.

Specimen-induced aberrations cause a reduction in signal levels and resolution in fluorescence microscopy. Aberrations also affect the image contrast achieved by these microscopes. We model the effects of aberrations on the fluorescence signals acquired from different specimen structures, such as po...

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Bibliographic Details
Main Authors: Simmonds, R, Wilson, T, Booth, M
Format: Journal article
Language:English
Published: 2012