Transfer of YACs up to 2.3 Mb intact into human cells with polyethylenimine.
The transfer of large YAC DNA into human cells is a laborious procedure. High quality pulsed field gel purified DNA is required, which is easily sheared during manipulation before transfection or degraded in the endosome of the cell following transfection. NaCl and polyamines compact and prevent DNA...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1999
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