Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch
The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to o...
| Main Authors: | , , , , , , |
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| Formato: | Journal article |
| Publicado em: |
AAAS
2010
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| _version_ | 1826274025872031744 |
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| author | Bai, F Branch, R Nicolau Jr, D Pilizota, T Steele, B Maini, P Berry, R |
| author_facet | Bai, F Branch, R Nicolau Jr, D Pilizota, T Steele, B Maini, P Berry, R |
| author_sort | Bai, F |
| collection | OXFORD |
| description | The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread—the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes. |
| first_indexed | 2024-03-06T22:37:11Z |
| format | Journal article |
| id | oxford-uuid:5a4a5cdd-694e-4ff4-955a-3e5f58fc7faf |
| institution | University of Oxford |
| last_indexed | 2024-03-06T22:37:11Z |
| publishDate | 2010 |
| publisher | AAAS |
| record_format | dspace |
| spelling | oxford-uuid:5a4a5cdd-694e-4ff4-955a-3e5f58fc7faf2022-03-26T17:14:58ZConformational spread as a mechanism for cooperativity in the bacterial flagellar switchJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5a4a5cdd-694e-4ff4-955a-3e5f58fc7fafMathematical Institute - ePrintsAAAS2010Bai, FBranch, RNicolau Jr, DPilizota, TSteele, BMaini, PBerry, RThe bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread—the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes. |
| spellingShingle | Bai, F Branch, R Nicolau Jr, D Pilizota, T Steele, B Maini, P Berry, R Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title | Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title_full | Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title_fullStr | Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title_full_unstemmed | Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title_short | Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
| title_sort | conformational spread as a mechanism for cooperativity in the bacterial flagellar switch |
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