An evaluation of Coomassie Brilliant Blue as a stain for quantitative microdensitometry of protein in section.
The specificity and stoichiometry of the binding of Coomassie Brilliant Blue (CBB) to protein in section has been examined using both frozen protein matrices and plant material. The maximum adsorbance of the stain, bound and in solution, was found to be 620 nm although variation in the results at th...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1978
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