Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disj...
| المؤلفون الرئيسيون: | , , , , , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2008
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| _version_ | 1826274088932343808 |
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| author | Pauli, A Althoff, F Oliveira, R Heidmann, S Schuldiner, O Lehner, C Dickson, B Nasmyth, K |
| author_facet | Pauli, A Althoff, F Oliveira, R Heidmann, S Schuldiner, O Lehner, C Dickson, B Nasmyth, K |
| author_sort | Pauli, A |
| collection | OXFORD |
| description | Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesin's function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa. © 2008 Elsevier Inc. All rights reserved. |
| first_indexed | 2024-03-06T22:38:09Z |
| format | Journal article |
| id | oxford-uuid:5a9fea48-b33e-4ea7-b263-ac9a985053d3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:38:09Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:5a9fea48-b33e-4ea7-b263-ac9a985053d32022-03-26T17:16:55ZCell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neuronsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5a9fea48-b33e-4ea7-b263-ac9a985053d3EnglishSymplectic Elements at Oxford2008Pauli, AAlthoff, FOliveira, RHeidmann, SSchuldiner, OLehner, CDickson, BNasmyth, KCohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesin's function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa. © 2008 Elsevier Inc. All rights reserved. |
| spellingShingle | Pauli, A Althoff, F Oliveira, R Heidmann, S Schuldiner, O Lehner, C Dickson, B Nasmyth, K Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title | Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title_full | Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title_fullStr | Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title_full_unstemmed | Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title_short | Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons |
| title_sort | cell type specific tev protease cleavage reveals cohesin functions in drosophila neurons |
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