Fluorescence-based active site probes for profiling deubiquitinating enzymes.
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating...
| Autors principals: | , , , , , |
|---|---|
| Format: | Journal article |
| Idioma: | English |
| Publicat: |
2012
|