Single-molecule imaging of electroporated dye-labelled CheY in live Escherichia coli

For the past two decades, the use of genetically fused fluorescent proteins (FPs) has greatly contributed to the study of chemotactic signalling in Escherichia coli including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach su...

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Main Authors: Di Paolo, D, Afanzar, O, Armitage, J, Berry, R
格式: Journal article
出版: Royal Society 2016