Monitoring multiple distances within a single molecule using switchable FRET.

The analysis of structure and dynamics of biomolecules is important for understanding their function. Toward this aim, we introduce a method called 'switchable FRET', which combines single-molecule fluorescence resonance energy transfer (FRET) with reversible photoswitching of fluorophores...

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Bibliographic Details
Main Authors: Uphoff, S, Holden, S, Le Reste, L, Periz, J, van de Linde, S, Heilemann, M, Kapanidis, A
Format: Journal article
Language:English
Published: 2010