Two-color far-field fluorescence nanoscopy based on photoswitchable emitters

We demonstrate two-color far-field fluorescence microscopy with nanoscale spatial resolution based on the photoswitching of individual fluorescent markers. By enabling, recording, and disabling the emission of the reversibly switchable fluorescent protein rsFastLime and of the organic fluorophore cy...

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Bibliographic Details
Main Authors: Bock, H, Geisler, C, Wurm, C, Von Middendorff, C, Jakobs, S, Schoenle, A, Egner, A, Hell, S, Eggeling, C
Format: Journal article
Language:English
Published: 2007