Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores.
Staphylococcal α-hemolysin (αHL) forms a heptameric pore that features a 14-stranded transmembrane β-barrel. We attempted to force the αHL pore to adopt novel stoichiometries by oligomerizing subunit dimers generated by in vitro transcription and translation of a tandem gene. However, in vitro trans...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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