Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores.

Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores.

Staphylococcal α-hemolysin (αHL) forms a heptameric pore that features a 14-stranded transmembrane β-barrel. We attempted to force the αHL pore to adopt novel stoichiometries by oligomerizing subunit dimers generated by in vitro transcription and translation of a tandem gene. However, in vitro trans...

書誌詳細
主要な著者:	Hammerstein, A, Jayasinghe, L, Bayley, H
フォーマット:	Journal article
言語:	English
出版事項:	2011

類似資料

Single-molecule chemistry studies with engineered alpha-hemolysin pores
著者:: Hammerstein, A
出版事項: (2011)

Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore.
著者:: Japrung, D, 等
出版事項: (2010)

Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge.
著者:: Maglia, G, 等
出版事項: (2008)

Translocating kilobase RNA through the Staphylococcal α-hemolysin nanopore.
著者:: Cracknell, J, 等
出版事項: (2013)

Subunit stoichiometry of staphylococcal alpha-hemolysin in crystals and on membranes: a heptameric transmembrane pore.
著者:: Gouaux, J, 等
出版事項: (1994)

Redirecting pore assembly of staphylococcal α-hemolysin by protein engineering
著者:: Koo, S, 等
出版事項: (2019)

Targeted protein degradation: expanding the toolbox
著者:: Schapira, M, 等
出版事項: (2019)

Protein detection by nanopores equipped with aptamers.
著者:: Rotem, D, 等
出版事項: (2012)

Interactions between residues in staphylococcal alpha-hemolysin revealed by reversion mutagenesis.
著者:: Panchal, R, 等
出版事項: (1995)

Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore.
著者:: Song, L, 等
出版事項: (1996)

Nucleobase recognition in ssDNA at the central constriction of the alpha-hemolysin pore.
著者:: Stoddart, D, 等
出版事項: (2010)

Assembly of the oligomeric membrane pore formed by Staphylococcal alpha-hemolysin examined by truncation mutagenesis.
著者:: Walker, B, 等
出版事項: (1992)

Expanding the ligation toolbox through protein engineering of peptide asparaginyl ligases (PALs)
著者:: Tan, Shaun Jun Hao
出版事項: (2023)

Engineering a dimeric transmembrane protein nanoconduit
著者:: Mantri, S, 等
出版事項: (2012)

Surface labeling of key residues during assembly of the transmembrane pore formed by staphylococcal alpha-hemolysin.
著者:: Krishnasastry, M, 等
出版事項: (1994)

The internal cavity of the staphylococcal alpha-hemolysin pore accommodates approximately 175 exogenous amino acid residues.
著者:: Jung, Y, 等
出版事項: (2005)

Expanding the CRISPR Toolbox for Engineering Lycopene Biosynthesis in Corynebacterium glutamicum
著者:: Zhan, Zhimin, 等
出版事項: (2024)

Visualization of multivalent binding events using α-hemolysin
著者:: Gregoir, P, 等
出版事項: (2020)

Spontaneous oligomerization of a staphylococcal alpha-hemolysin conformationally constrained by removal of residues that form the transmembrane beta-barrel.
著者:: Cheley, S, 等
出版事項: (1997)

Model-based prediction of the alpha-hemolysin structure in the hexameric state.
著者:: Furini, S, 等
出版事項: (2008)

Imaging the assembly of the Staphylococcal pore-forming toxin alpha-Hemolysin
著者:: Thompson, J, 等
出版事項: (2009)

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins
著者:: Mantri, S
出版事項: (2013)

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins
著者:: Mantri, S
出版事項: (2013)

Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.
著者:: Banerjee, A, 等
出版事項: (2010)

Developing an expanded vector control toolbox for malaria elimination
著者:: Tusting, L, 等
出版事項: (2017)

Properties of Bacillus cereus hemolysin II: a heptameric transmembrane pore.
著者:: Miles, G, 等
出版事項: (2002)

Expanding the low-dimensional interface engineering toolbox for efficient perovskite solar cells
著者:: Ye, Senyun, 等
出版事項: (2023)

An engineered dimeric protein pore that spans adjacent lipid bilayers.
著者:: Mantri, S, 等
出版事項: (2013)

Continuous base identification for single-molecule nanopore DNA sequencing
著者:: Clarke, J, 等
出版事項: (2009)

Continuous base identification for single-molecule nanopore DNA sequencing.
著者:: Clarke, J, 等
出版事項: (2009)

Key residues for membrane binding, oligomerization, and pore forming activity of staphylococcal alpha-hemolysin identified by cysteine scanning mutagenesis and targeted chemical modification.
著者:: Walker, B, 等
出版事項: (1995)

Single-molecule protein phosphorylation and dephosphorylation by nanopore enzymology
著者:: Harrington, L, 等
出版事項: (2018)

Multistep protein unfolding during nanopore translocation.
著者:: Rodriguez-Larrea, D, 等
出版事項: (2013)

Multistep protein unfolding during nanopore translocation
著者:: Rodriguez-Larrea, D, 等
出版事項: (2013)

Fluorinated amphiphiles control the insertion of α-hemolysin pores into lipid bilayers.
著者:: Raychaudhuri, P, 等
出版事項: (2011)

Protein nanopores with covalently attached molecular adapters.
著者:: Wu, H, 等
出版事項: (2007)

Controlled translocation of individual DNA molecules through protein nanopores with engineered molecular brakes.
著者:: Rincon-Restrepo, M, 等
出版事項: (2011)

Engineering a Biomimetic Biological Nanopore to Selectively Capture Folded Target Proteins
著者:: Maglia, G, 等
出版事項: (2013)

Analysis of single nucleic acid molecules with protein nanopores.
著者:: Maglia, G, 等
出版事項: (2010)

Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity.
著者:: Walker, B, 等
出版事項: (1992)