Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation
Cytochrome P450 CYP101A1 (P450cam) hydroxylates camphor by receiving two distinct electrons from its unique reductase, putidaredoxin (Pdx). Upon binding ferric P450cam, Pdx is now known to trigger a conformational change in the enzyme. This Pdx-induced conversion may provide the trigger to coordinat...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2017
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| _version_ | 1797071950348025856 |
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| author | Liou, S Myers, W Oswald, J Britt, R Goodin, D |
| author_facet | Liou, S Myers, W Oswald, J Britt, R Goodin, D |
| author_sort | Liou, S |
| collection | OXFORD |
| description | Cytochrome P450 CYP101A1 (P450cam) hydroxylates camphor by receiving two distinct electrons from its unique reductase, putidaredoxin (Pdx). Upon binding ferric P450cam, Pdx is now known to trigger a conformational change in the enzyme. This Pdx-induced conversion may provide the trigger to coordinate enzyme turnover and protect the enzyme from oxidative damage, so the interactions responsible for this conversion are of significant interest at present. This proposed role for Pdx requires that its interactions with P450cam be different for the open and closed conformations. In this study, we show that the binding thermodynamics of Pdx does indeed differ in the predicted way when the conformation of P450cam is held in different states. However, double electron–electron resonance measurements of intermolecular distances in the Pdx/P450cam complex show that the geometry of the complex is nearly identical for the open and closed states of P450cam. These studies show that Pdx appears to make a single distinct interaction with its binding site on the enzyme and triggers the conformational change through very subtle structural interactions. |
| first_indexed | 2024-03-06T23:00:32Z |
| format | Journal article |
| id | oxford-uuid:61f1a413-e841-452d-bdc4-8f4f1c046bd9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:00:32Z |
| publishDate | 2017 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:61f1a413-e841-452d-bdc4-8f4f1c046bd92022-03-26T18:03:13ZPutidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:61f1a413-e841-452d-bdc4-8f4f1c046bd9EnglishSymplectic Elements at OxfordAmerican Chemical Society2017Liou, SMyers, WOswald, JBritt, RGoodin, DCytochrome P450 CYP101A1 (P450cam) hydroxylates camphor by receiving two distinct electrons from its unique reductase, putidaredoxin (Pdx). Upon binding ferric P450cam, Pdx is now known to trigger a conformational change in the enzyme. This Pdx-induced conversion may provide the trigger to coordinate enzyme turnover and protect the enzyme from oxidative damage, so the interactions responsible for this conversion are of significant interest at present. This proposed role for Pdx requires that its interactions with P450cam be different for the open and closed conformations. In this study, we show that the binding thermodynamics of Pdx does indeed differ in the predicted way when the conformation of P450cam is held in different states. However, double electron–electron resonance measurements of intermolecular distances in the Pdx/P450cam complex show that the geometry of the complex is nearly identical for the open and closed states of P450cam. These studies show that Pdx appears to make a single distinct interaction with its binding site on the enzyme and triggers the conformational change through very subtle structural interactions. |
| spellingShingle | Liou, S Myers, W Oswald, J Britt, R Goodin, D Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title | Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title_full | Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title_fullStr | Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title_full_unstemmed | Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title_short | Putidaredoxin binds to the same site on cytochrome P450cam in the open and closed conformation |
| title_sort | putidaredoxin binds to the same site on cytochrome p450cam in the open and closed conformation |
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