Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion.
In intact sheep gonadotropes, the protein kinase inhibitor, staurosporine, inhibited the stimulatory effect of phorbol 12-myristate 13-acetate (PMA) on luteinizing hormone (LH) secretion. Under the same conditions staurosporine enhanced gonadotrophin-releasing hormone (GnRH)-stimulated LH exocytosis...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1990
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| _version_ | 1826275676747988992 |
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| author | Van Der Merwe, P Millar, R Wakefield, I Macrae, M Davidson, J |
| author_facet | Van Der Merwe, P Millar, R Wakefield, I Macrae, M Davidson, J |
| author_sort | Van Der Merwe, P |
| collection | OXFORD |
| description | In intact sheep gonadotropes, the protein kinase inhibitor, staurosporine, inhibited the stimulatory effect of phorbol 12-myristate 13-acetate (PMA) on luteinizing hormone (LH) secretion. Under the same conditions staurosporine enhanced gonadotrophin-releasing hormone (GnRH)-stimulated LH exocytosis without altering the EC50 of GnRH and without affecting basal LH exocytosis. These results suggest that PKC does not play a major role in mediating acute GnRH-stimulated LH exocytosis. Furthermore, they demonstrate that staurosporine enhances GnRH stimulus-secretion coupling. Both extracellular Ca2(+)-dependent and Ca2(+)-independent components of GnRH-stimulated LH secretion were enhanced by the drug. Staurosporine had no effect on GnRH stimulation of cAMP and inositol phosphate synthesis. In permeabilized cells staurosporine did not enhance Ca2(+)- and cAMP-stimulated LH exocytosis. Based on these results we hypothesize that staurosporine inhibits a protein kinase which is activated by GnRH and which negatively modulates GnRH stimulus-secretion coupling. |
| first_indexed | 2024-03-06T23:02:24Z |
| format | Journal article |
| id | oxford-uuid:62990c3d-e276-4344-999b-8921962539b1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:02:24Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:62990c3d-e276-4344-999b-8921962539b12022-03-26T18:07:19ZStaurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:62990c3d-e276-4344-999b-8921962539b1EnglishSymplectic Elements at Oxford1990Van Der Merwe, PMillar, RWakefield, IMacrae, MDavidson, JIn intact sheep gonadotropes, the protein kinase inhibitor, staurosporine, inhibited the stimulatory effect of phorbol 12-myristate 13-acetate (PMA) on luteinizing hormone (LH) secretion. Under the same conditions staurosporine enhanced gonadotrophin-releasing hormone (GnRH)-stimulated LH exocytosis without altering the EC50 of GnRH and without affecting basal LH exocytosis. These results suggest that PKC does not play a major role in mediating acute GnRH-stimulated LH exocytosis. Furthermore, they demonstrate that staurosporine enhances GnRH stimulus-secretion coupling. Both extracellular Ca2(+)-dependent and Ca2(+)-independent components of GnRH-stimulated LH secretion were enhanced by the drug. Staurosporine had no effect on GnRH stimulation of cAMP and inositol phosphate synthesis. In permeabilized cells staurosporine did not enhance Ca2(+)- and cAMP-stimulated LH exocytosis. Based on these results we hypothesize that staurosporine inhibits a protein kinase which is activated by GnRH and which negatively modulates GnRH stimulus-secretion coupling. |
| spellingShingle | Van Der Merwe, P Millar, R Wakefield, I Macrae, M Davidson, J Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title | Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title_full | Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title_fullStr | Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title_full_unstemmed | Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title_short | Staurosporine enhances gonadotrophin-releasing hormone-stimulated luteinizing hormone secretion. |
| title_sort | staurosporine enhances gonadotrophin releasing hormone stimulated luteinizing hormone secretion |
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