Conformational changes of the H+-ATPase from Escherichia coli upon nucleotide binding detected by single molecule fluorescence.

Using a confocal fluorescence microscope with an avalanche photodiode as detector, we studied the fluorescence of the tetramethylrhodamine labeled F1 part of the H+-ATPase from Escherichia coli, EF1, carrying the gammaT106-C mutation [Aggeler, J.A. and Capaldi, R.A. (1992) J. Biol. Chem. 267, 21355-...

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Bibliographic Details
Main Authors: Börsch, M, Turina, P, Eggeling, C, Fries, JR, Seidel, C, Labahn, A, Gräber, P
Format: Journal article
Language:English
Published: 1998