Mycobacterial DNA extraction for whole-genome sequencing from early positive liquid (MGIT) cultures

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA ext...

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Bibliographic Details
Main Authors: Votintseva, A, Pankhurst, L, Anson, L, Morgan, MR, Gascoyne-Binzi, D, Walker, T, Quan, T, Wyllie, D, Del Ojo Elias, C, Wilcox, M, Walker, A, Peto, T, Crook, D
Format: Journal article
Language:English
Published: American Society for Microbiology 2015
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