The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.
In this chapter, protocols for the construction of expression vectors using In-Fusion PCR cloning are presented. The method enables vector and insert DNA sequences to be seamlessly joined in a ligation-independent reaction. This property of the In-Fusion process has been exploited in the design of a...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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