The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.
In this chapter, protocols for the construction of expression vectors using In-Fusion PCR cloning are presented. The method enables vector and insert DNA sequences to be seamlessly joined in a ligation-independent reaction. This property of the In-Fusion process has been exploited in the design of a...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1826276164873748480 |
|---|---|
| author | Berrow, N Alderton, D Owens, R |
| author_facet | Berrow, N Alderton, D Owens, R |
| author_sort | Berrow, N |
| collection | OXFORD |
| description | In this chapter, protocols for the construction of expression vectors using In-Fusion PCR cloning are presented. The method enables vector and insert DNA sequences to be seamlessly joined in a ligation-independent reaction. This property of the In-Fusion process has been exploited in the design of a suite of multi-host compatible vectors for the expression of proteins with precisely engineered His-tags. Vector preparation, PCR amplification of the sequence to be cloned and the procedure for inserting the PCR product into the vector by In-Fusion are described. |
| first_indexed | 2024-03-06T23:09:53Z |
| format | Journal article |
| id | oxford-uuid:65156a16-d189-4b63-895a-0e9699ec036e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:09:53Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:65156a16-d189-4b63-895a-0e9699ec036e2022-03-26T18:23:17ZThe precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:65156a16-d189-4b63-895a-0e9699ec036eEnglishSymplectic Elements at Oxford2009Berrow, NAlderton, DOwens, RIn this chapter, protocols for the construction of expression vectors using In-Fusion PCR cloning are presented. The method enables vector and insert DNA sequences to be seamlessly joined in a ligation-independent reaction. This property of the In-Fusion process has been exploited in the design of a suite of multi-host compatible vectors for the expression of proteins with precisely engineered His-tags. Vector preparation, PCR amplification of the sequence to be cloned and the procedure for inserting the PCR product into the vector by In-Fusion are described. |
| spellingShingle | Berrow, N Alderton, D Owens, R The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title | The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title_full | The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title_fullStr | The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title_full_unstemmed | The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title_short | The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. |
| title_sort | precise engineering of expression vectors using high throughput in fusion pcr cloning |
| work_keys_str_mv | AT berrown thepreciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning AT aldertond thepreciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning AT owensr thepreciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning AT berrown preciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning AT aldertond preciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning AT owensr preciseengineeringofexpressionvectorsusinghighthroughputinfusionpcrcloning |