Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning
Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized i...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Frontiers Media
2022
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| _version_ | 1797106908373450752 |
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| author | Wu, X Xing, X Wang, M Feng, Y Wietek, N Chong, K El-Sahhar, S Ahmed, AA Zang, R Zeng, Y |
| author_facet | Wu, X Xing, X Wang, M Feng, Y Wietek, N Chong, K El-Sahhar, S Ahmed, AA Zang, R Zeng, Y |
| author_sort | Wu, X |
| collection | OXFORD |
| description | Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized. |
| first_indexed | 2024-03-07T07:09:11Z |
| format | Journal article |
| id | oxford-uuid:66af98db-4bef-4fb8-bb36-dee26204042f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:09:11Z |
| publishDate | 2022 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:66af98db-4bef-4fb8-bb36-dee26204042f2022-06-07T15:45:35ZInvestigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learningJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:66af98db-4bef-4fb8-bb36-dee26204042fEnglishSymplectic ElementsFrontiers Media2022Wu, XXing, XWang, MFeng, YWietek, NChong, KEl-Sahhar, SAhmed, AAZang, RZeng, YIncreasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized. |
| spellingShingle | Wu, X Xing, X Wang, M Feng, Y Wietek, N Chong, K El-Sahhar, S Ahmed, AA Zang, R Zeng, Y Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title | Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title_full | Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title_fullStr | Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title_full_unstemmed | Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title_short | Investigation of the potential mechanisms underlying nuclear F-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning |
| title_sort | investigation of the potential mechanisms underlying nuclear f actin organization in ovarian cancer cells by high throughput screening in combination with deep learning |
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