Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells.
How different extracellular stimuli can evoke different spatiotemporal Ca2+ signals is uncertain. We have elucidated a novel paradigm whereby different agonists use different Ca2+-storing organelles ("organelle selection") to evoke unique responses. Some agonists select the endoplasmic ret...
| Huvudupphovsmän: | , , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2004
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| _version_ | 1826276959582158848 |
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| author | Yamasaki, M Masgrau, R Morgan, A Churchill, G Patel, S Ashcroft, S Galione, A |
| author_facet | Yamasaki, M Masgrau, R Morgan, A Churchill, G Patel, S Ashcroft, S Galione, A |
| author_sort | Yamasaki, M |
| collection | OXFORD |
| description | How different extracellular stimuli can evoke different spatiotemporal Ca2+ signals is uncertain. We have elucidated a novel paradigm whereby different agonists use different Ca2+-storing organelles ("organelle selection") to evoke unique responses. Some agonists select the endoplasmic reticulum (ER), and others select lysosome-related (acidic) organelles, evoking spatial Ca2+ responses that mirror the organellar distribution. In pancreatic acinar cells, acetylcholine and bombesin exclusively select the ER Ca2+ store, whereas cholecystokinin additionally recruits a lysosome-related organelle. Similarly, in a pancreatic beta cell line MIN6, acetylcholine selects only the ER, whereas glucose mobilizes Ca2+ from a lysosome-related organelle. We also show that the key to organelle selection is the agonist-specific coupling messenger(s) such that the ER is selected by recruitment of inositol 1,4,5-trisphosphate (or cADP-ribose), whereas lysosome-related organelles are selected by NAADP. |
| first_indexed | 2024-03-06T23:21:41Z |
| format | Journal article |
| id | oxford-uuid:68ee80cf-ffac-48d8-8790-00a6d4795a70 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:21:41Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:68ee80cf-ffac-48d8-8790-00a6d4795a702022-03-26T18:48:15ZOrganelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:68ee80cf-ffac-48d8-8790-00a6d4795a70EnglishSymplectic Elements at Oxford2004Yamasaki, MMasgrau, RMorgan, AChurchill, GPatel, SAshcroft, SGalione, AHow different extracellular stimuli can evoke different spatiotemporal Ca2+ signals is uncertain. We have elucidated a novel paradigm whereby different agonists use different Ca2+-storing organelles ("organelle selection") to evoke unique responses. Some agonists select the endoplasmic reticulum (ER), and others select lysosome-related (acidic) organelles, evoking spatial Ca2+ responses that mirror the organellar distribution. In pancreatic acinar cells, acetylcholine and bombesin exclusively select the ER Ca2+ store, whereas cholecystokinin additionally recruits a lysosome-related organelle. Similarly, in a pancreatic beta cell line MIN6, acetylcholine selects only the ER, whereas glucose mobilizes Ca2+ from a lysosome-related organelle. We also show that the key to organelle selection is the agonist-specific coupling messenger(s) such that the ER is selected by recruitment of inositol 1,4,5-trisphosphate (or cADP-ribose), whereas lysosome-related organelles are selected by NAADP. |
| spellingShingle | Yamasaki, M Masgrau, R Morgan, A Churchill, G Patel, S Ashcroft, S Galione, A Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title | Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title_full | Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title_fullStr | Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title_full_unstemmed | Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title_short | Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells. |
| title_sort | organelle selection determines agonist specific ca2 signals in pancreatic acinar and beta cells |
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