A genetically encoded, fluorescent indicator for cyclic AMP in living cells.

Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells. By tagging the cAMP effector protein kinase A...

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Bibliographic Details
Main Authors: Zaccolo, M, De Giorgi, F, Cho, C, Feng, L, Knapp, T, Negulescu, P, Taylor, S, Tsien, R, Pozzan, T
Format: Journal article
Language:English
Published: 2000