Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina
Although adeno-associated viral (AAV) vector-mediated retinal gene therapies have demonstrated efficacy, the mechanisms underlying dose-dependent retinal inflammation remain poorly understood. Here we present a quantitative analysis of cellular immune response to subretinal AAV gene therapy in mice...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2021
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| _version_ | 1826277081805225984 |
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| author | Chandler, LC McClements, ME Yusuf, IH Martinez-Fernandez de la Camara, C MacLaren, RE Xue, K |
| author_facet | Chandler, LC McClements, ME Yusuf, IH Martinez-Fernandez de la Camara, C MacLaren, RE Xue, K |
| author_sort | Chandler, LC |
| collection | OXFORD |
| description | Although adeno-associated viral (AAV) vector-mediated retinal gene therapies have demonstrated efficacy, the mechanisms underlying dose-dependent retinal inflammation remain poorly understood. Here we present a quantitative analysis of cellular immune response to subretinal AAV gene therapy in mice using multicolor flow cytometry with a panel of key immune cell markers. A significant increase in CD45+ retinal leukocytes was detected from day 14 post-subretinal injection of an AAV8 vector (1x109 genome copies) encoding green fluorescent protein (GFP) driven by a ubiquitous promoter. These predominantly consisted of infiltrating peripheral leukocytes including macrophages, natural killer cells, CD4 and CD8 T cells, and natural killer T cells, no significant change in resident microglia population was detected. This cellular response was persistent at 28 days and suggestive of type 1 cell-mediated effector immunity. High levels (∼80%) of GFP fluorescence were found in the microglia, implicating their role in viral antigen presentation and peripheral leukocyte recruitment. When compared against AAV.GFP in paired eyes, an equivalent dose of an otherwise identical vector encoding the human therapeutic transgene Rab-escort protein 1 (REP1) elicited a significantly diminished cellular immune response (4.2-fold; p=0.0221). However, the distribution of immune cell populations remained similar, indicating a common mechanism of AAV-induced immune activation.
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| first_indexed | 2024-03-06T23:23:32Z |
| format | Journal article |
| id | oxford-uuid:6998afe6-9ac3-41fb-a0d4-c0da0926b107 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:23:32Z |
| publishDate | 2021 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:6998afe6-9ac3-41fb-a0d4-c0da0926b1072022-03-26T18:51:58ZCharacterizing the cellular immune response to subretinal AAV gene therapy in the murine retinaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6998afe6-9ac3-41fb-a0d4-c0da0926b107EnglishSymplectic ElementsCell Press2021Chandler, LCMcClements, MEYusuf, IHMartinez-Fernandez de la Camara, CMacLaren, REXue, KAlthough adeno-associated viral (AAV) vector-mediated retinal gene therapies have demonstrated efficacy, the mechanisms underlying dose-dependent retinal inflammation remain poorly understood. Here we present a quantitative analysis of cellular immune response to subretinal AAV gene therapy in mice using multicolor flow cytometry with a panel of key immune cell markers. A significant increase in CD45+ retinal leukocytes was detected from day 14 post-subretinal injection of an AAV8 vector (1x109 genome copies) encoding green fluorescent protein (GFP) driven by a ubiquitous promoter. These predominantly consisted of infiltrating peripheral leukocytes including macrophages, natural killer cells, CD4 and CD8 T cells, and natural killer T cells, no significant change in resident microglia population was detected. This cellular response was persistent at 28 days and suggestive of type 1 cell-mediated effector immunity. High levels (∼80%) of GFP fluorescence were found in the microglia, implicating their role in viral antigen presentation and peripheral leukocyte recruitment. When compared against AAV.GFP in paired eyes, an equivalent dose of an otherwise identical vector encoding the human therapeutic transgene Rab-escort protein 1 (REP1) elicited a significantly diminished cellular immune response (4.2-fold; p=0.0221). However, the distribution of immune cell populations remained similar, indicating a common mechanism of AAV-induced immune activation. |
| spellingShingle | Chandler, LC McClements, ME Yusuf, IH Martinez-Fernandez de la Camara, C MacLaren, RE Xue, K Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title | Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title_full | Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title_fullStr | Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title_full_unstemmed | Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title_short | Characterizing the cellular immune response to subretinal AAV gene therapy in the murine retina |
| title_sort | characterizing the cellular immune response to subretinal aav gene therapy in the murine retina |
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