Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.
Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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