Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.
Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2011
|
| _version_ | 1826277095197638656 |
|---|---|
| author | Shelbourne, M Chen, X Brown, T El-Sagheer, A |
| author_facet | Shelbourne, M Chen, X Brown, T El-Sagheer, A |
| author_sort | Shelbourne, M |
| collection | OXFORD |
| description | Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis. |
| first_indexed | 2024-03-06T23:23:44Z |
| format | Journal article |
| id | oxford-uuid:69aa415a-ee5a-4461-be60-39bcb2e87743 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:23:44Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:69aa415a-ee5a-4461-be60-39bcb2e877432022-03-26T18:52:24ZFast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:69aa415a-ee5a-4461-be60-39bcb2e87743EnglishSymplectic Elements at Oxford2011Shelbourne, MChen, XBrown, TEl-Sagheer, ATemplated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis. |
| spellingShingle | Shelbourne, M Chen, X Brown, T El-Sagheer, A Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title | Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title_full | Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title_fullStr | Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title_full_unstemmed | Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title_short | Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction. |
| title_sort | fast copper free click dna ligation by the ring strain promoted alkyne azide cycloaddition reaction |
| work_keys_str_mv | AT shelbournem fastcopperfreeclickdnaligationbytheringstrainpromotedalkyneazidecycloadditionreaction AT chenx fastcopperfreeclickdnaligationbytheringstrainpromotedalkyneazidecycloadditionreaction AT brownt fastcopperfreeclickdnaligationbytheringstrainpromotedalkyneazidecycloadditionreaction AT elsagheera fastcopperfreeclickdnaligationbytheringstrainpromotedalkyneazidecycloadditionreaction |