Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission.
Transmission of HIV-1 via intercellular connections has been estimated as 100-1000 times more efficient than a cell-free process, perhaps in part explaining persistent viral spread in the presence of neutralizing antibodies. Such effective intercellular transfer of HIV-1 could occur through virologi...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2008
|
| _version_ | 1797074442126360576 |
|---|---|
| author | Sowinski, S Jolly, C Berninghausen, O Purbhoo, M Chauveau, A Köhler, K Oddos, S Eissmann, P Brodsky, F Hopkins, C Onfelt, B Sattentau, Q Davis, D |
| author_facet | Sowinski, S Jolly, C Berninghausen, O Purbhoo, M Chauveau, A Köhler, K Oddos, S Eissmann, P Brodsky, F Hopkins, C Onfelt, B Sattentau, Q Davis, D |
| author_sort | Sowinski, S |
| collection | OXFORD |
| description | Transmission of HIV-1 via intercellular connections has been estimated as 100-1000 times more efficient than a cell-free process, perhaps in part explaining persistent viral spread in the presence of neutralizing antibodies. Such effective intercellular transfer of HIV-1 could occur through virological synapses or target-cell filopodia connected to infected cells. Here we report that membrane nanotubes, formed when T cells make contact and subsequently part, provide a new route for HIV-1 transmission. Membrane nanotubes are known to connect various cell types, including neuronal and immune cells, and allow calcium-mediated signals to spread between connected myeloid cells. However, T-cell nanotubes are distinct from open-ended membranous tethers between other cell types, as a dynamic junction persists within T-cell nanotubes or at their contact with cell bodies. We also report that an extracellular matrix scaffold allows T-cell nanotubes to adopt variably shaped contours. HIV-1 transfers to uninfected T cells through nanotubes in a receptor-dependent manner. These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize. |
| first_indexed | 2024-03-06T23:36:13Z |
| format | Journal article |
| id | oxford-uuid:6dc2330b-8444-4e56-96b7-546eb72b143f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T23:36:13Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:6dc2330b-8444-4e56-96b7-546eb72b143f2022-03-26T19:19:48ZMembrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:6dc2330b-8444-4e56-96b7-546eb72b143fEnglishSymplectic Elements at Oxford2008Sowinski, SJolly, CBerninghausen, OPurbhoo, MChauveau, AKöhler, KOddos, SEissmann, PBrodsky, FHopkins, COnfelt, BSattentau, QDavis, DTransmission of HIV-1 via intercellular connections has been estimated as 100-1000 times more efficient than a cell-free process, perhaps in part explaining persistent viral spread in the presence of neutralizing antibodies. Such effective intercellular transfer of HIV-1 could occur through virological synapses or target-cell filopodia connected to infected cells. Here we report that membrane nanotubes, formed when T cells make contact and subsequently part, provide a new route for HIV-1 transmission. Membrane nanotubes are known to connect various cell types, including neuronal and immune cells, and allow calcium-mediated signals to spread between connected myeloid cells. However, T-cell nanotubes are distinct from open-ended membranous tethers between other cell types, as a dynamic junction persists within T-cell nanotubes or at their contact with cell bodies. We also report that an extracellular matrix scaffold allows T-cell nanotubes to adopt variably shaped contours. HIV-1 transfers to uninfected T cells through nanotubes in a receptor-dependent manner. These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize. |
| spellingShingle | Sowinski, S Jolly, C Berninghausen, O Purbhoo, M Chauveau, A Köhler, K Oddos, S Eissmann, P Brodsky, F Hopkins, C Onfelt, B Sattentau, Q Davis, D Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title | Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title_full | Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title_fullStr | Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title_full_unstemmed | Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title_short | Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. |
| title_sort | membrane nanotubes physically connect t cells over long distances presenting a novel route for hiv 1 transmission |
| work_keys_str_mv | AT sowinskis membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT jollyc membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT berninghauseno membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT purbhoom membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT chauveaua membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT kohlerk membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT oddoss membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT eissmannp membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT brodskyf membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT hopkinsc membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT onfeltb membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT sattentauq membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission AT davisd membranenanotubesphysicallyconnecttcellsoverlongdistancespresentinganovelrouteforhiv1transmission |