Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes.
Biological membranes decorated with suitable contrast agents give rise to nonlinear optical signals such as two-photon fluorescence and harmonic up-conversion when illuminated with ultra-short, high-intensity pulses of infrared laser light. Microscopic images based on these nonlinear contrasts were...
Main Authors: | Roorda, R, Hohl, T, Toledo-Crow, R, Miesenböck, G |
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Format: | Journal article |
Language: | English |
Published: |
2004
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