Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor.

We used the vapor phase of acrolein as an anhydrous fixative for timothy grass pollen in an immunogold double-labeling localization study of two different major allergens, Phl p I and Phl p V. More than 48 hr of fixation were needed for the subcellular pollen structures to be satisfactorily stabiliz...

وصف كامل

التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Grote, M, Dolecek, C, Van Ree, R, Valenta, R
التنسيق: Journal article
اللغة:English
منشور في: 1994