Global and pixel kinetic data analysis for FRET detection by multi-photon time-domain FLIM
FLIM/FRET is an extremely powerful technique that can microscopically locate nanometre-scale protein-protein interactions within live or fixed cells, both in vitro and in vivo. The key to performing sensitive FRET, via FLIM, besides the use of appropriate fluorophores, is the analysis of the time-re...
| Main Authors: | , , , , , |
|---|---|
| Format: | Conference item |
| Published: |
2005
|