In situ assay for analyzing the chromatin binding of proteins in fission yeast.

An in situ technique for studying the chromatin binding of proteins in single fission yeast cells (Schizosaccharomyces pombe) is described. Cells are permeabilized by enzymatic digestion and extracted with a detergent-containing buffer. This procedure removes soluble proteins, but proteins that are...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Kearsey, S, Brimage, L, Namdar, M, Ralph, E, Yang, X
Формат: Journal article
Хэл сонгох:English
Хэвлэсэн: 2005