Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.

Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.

Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates...

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Bibliografiske detaljer
Main Authors:	Bardelli, M, Zarate-Perez, F, Agundez, L, Jolinon, N, Michael Linden, R, Escalante, C, Henckaerts, E
Format:	Journal article
Sprog:	English
Udgivet:	Bio-Protocol 2017

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