Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.

Analysis of replicative intermediates of adeno-associated virus through hirt extraction and southern blotting.

Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates...

Descrizione completa

Dettagli Bibliografici
Autori principali:	Bardelli, M, Zarate-Perez, F, Agundez, L, Jolinon, N, Michael Linden, R, Escalante, C, Henckaerts, E
Natura:	Journal article
Lingua:	English
Pubblicazione:	Bio-Protocol 2017

Documenti analoghi

Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting
di: Martino Bardelli, et al.
Pubblicazione: (2017-05-01)

Identification of a functionally relevant adeno-associated virus Rep68 oligomeric interface.
di: Bardelli, M, et al.
Pubblicazione: (2016)

Determination of adeno-associated virus rep DNA binding using fluorescence anisotropy.
di: Zarate-Perez, F, et al.
Pubblicazione: (2017)

Determination of Adeno-associated Virus Rep DNA Binding Using Fluorescence Anisotropy
di: Francisco Zarate-Perez, et al.
Pubblicazione: (2017-03-01)

Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus
di: Agúndez, L, et al.
Pubblicazione: (2018)

Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus.
di: Leticia Agúndez, et al.
Pubblicazione: (2018-01-01)

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats
di: Karl Petri, et al.
Pubblicazione: (2014-05-01)

Nachwuchsförderpreis der SGBF 2022 an Carmen N. Hirt
di: Doris Edelmann
Pubblicazione: (2023-12-01)

The effect of DNA-dependent protein kinase on adeno-associated virus replication.
di: Young-Kook Choi, et al.
Pubblicazione: (2010-12-01)

„Der Herr ist mein Hirte“ – Konzeptuelle Metapher als Lebenswirklichkeit
di: Jakob Böckle
Pubblicazione: (2021-05-01)

Modified Hirt Procedure for Rapid Purification of Extrachromosomal DNA from Mammalian Cells
di: Uri Arad
Pubblicazione: (1998-05-01)

The art of blotting: Alexander Cozens's New Method as intermedial practice
di: Kaes, F
Pubblicazione: (2024)

Adeno-associated virus receptor complexes and implications for adeno-associated virus immune neutralization
di: Edward E. Large, et al.
Pubblicazione: (2023-02-01)

The X gene of adeno-associated virus 2 (AAV2) is involved in viral DNA replication.
di: Maohua Cao, et al.
Pubblicazione: (2014-01-01)

Definition of herpes simplex virus type 1 helper activities for adeno-associated virus early replication events.
di: Nathalie Alazard-Dany, et al.
Pubblicazione: (2009-03-01)

Evaluating strategies to normalise biological replicates of Western blot data.
di: Andrea Degasperi, et al.
Pubblicazione: (2014-01-01)

Southern Blotting of Long-Term Preserved DNA
di: Marc-André Laniel, et al.
Pubblicazione: (1997-04-01)

Automated design of genomic Southern blot probes
di: Komiyama Noboru H, et al.
Pubblicazione: (2010-01-01)

Model suggesting that replication of influenza virus is regulated by stabilization of replicative intermediates.
di: Vreede, F, et al.
Pubblicazione: (2004)

High intensity resistance training (HIRT) improves functional and immunological lung responses in elderly with metabolic syndrome
di: Rodolfo P. Vieira, et al.
Pubblicazione: (2020-08-01)

Jesus – der Herrscher als Hirte. Eine motivkritische Spurensuche zu Joh 10,1–18
di: Hanna-Maria Mehring
Pubblicazione: (2018-06-01)

Infrared exploration of the architectural heritage: from passive infrared thermography to hybrid infrared thermography (HIRT) approach
di: S. Sfarra, et al.
Pubblicazione: (2016-09-01)

In Vivo Modelling of Hepatitis B Virus Subgenotype A1 Replication Using Adeno-Associated Viral Vectors
di: Shonisani Wendy Limani, et al.
Pubblicazione: (2021-11-01)

Detection of Southern Rice Black-Streaked Dwarf Virus Using Western Blotting With P6
di: Xin Xie, et al.
Pubblicazione: (2021-01-01)

Virus Binding and Internalization Assay for Adeno-associated Virus
di: Garrett Berry, et al.
Pubblicazione: (2017-01-01)

Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights
di: Estrella Lopez-Gordo, et al.
Pubblicazione: (2024-03-01)

Virus Overlay Assay (Far-Western blotting)
di: Kun-Tong Jia, et al.
Pubblicazione: (2013-10-01)

Adeno-Associated Virus Monoinfection Induces a DNA Damage Response and DNA Repair That Contributes to Viral DNA Replication
di: Kang Ning, et al.
Pubblicazione: (2023-02-01)

High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals
di: Paoli Antonio, et al.
Pubblicazione: (2012-11-01)

In vitro Drug Susceptibility Assay for HBV Using Southern Blotting
di: Sung Ahn, et al.
Pubblicazione: (2015-04-01)

Adeno-associated virus vectors for gene therapy /
di: Flotte, T. R., et al.
Pubblicazione: (2005)

Advanced biomanufacturing and evaluation of adeno-associated virus
di: Kai Chen, et al.
Pubblicazione: (2024-02-01)

Fluorescence Microscopy in Adeno-Associated Virus Research
di: Susanne K. Golm, et al.
Pubblicazione: (2023-05-01)

Process Improvement of Adeno-Associated Virus Production
di: Jia-Shiung Guan, et al.
Pubblicazione: (2022-01-01)

Pharmacology of Recombinant Adeno-associated Virus Production
di: Magalie Penaud-Budloo, et al.
Pubblicazione: (2018-03-01)

The Interplay between Adeno-Associated Virus and Its Helper Viruses
di: Anita F. Meier, et al.
Pubblicazione: (2020-06-01)

Adeno-associated virus: from defective virus to effective vector
di: Gonçalves Manuel AFV
Pubblicazione: (2005-05-01)

Production and characterization of novel recombinant adeno-associated virus replicative-form genomes: a eukaryotic source of DNA for gene transfer.
di: Lina Li, et al.
Pubblicazione: (2013-01-01)

PCR, Southern Blotting and Oocyte Expression to Assess the Efficacy of Subtractive Hybridization
di: Douglas M. Silverstein, et al.
Pubblicazione: (1996-12-01)

Preparation of Parasite Protein Extracts and Western Blot Analysis
di: Arlett Heiber, et al.
Pubblicazione: (2014-06-01)