Microscope calibration using laser written fluorescence

Microscope calibration using laser written fluorescence

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written...

詳細記述

書誌詳細
主要な著者: Corbett, A, Shaw, M, Yacoot, A, Jefferson, A, Schermelleh, L, Wilson, T, Booth, M, Salter, P
フォーマット: Journal article
出版事項: Optical Society of America 2018
その他の書誌記述
要約:There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and threedimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.

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