Recognition of GT mismatches by Vsr mismatch endonuclease.
The Vsr mismatch endonuclease recognises the sequence CTWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5'-side of the mispaired thymine. By using base analogues of G and T we have explored the functional groups on the mismatch pair whic...
| Huvudupphovsmän: | , , , |
|---|---|
| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2000
|
| _version_ | 1826279752205336576 |
|---|---|
| author | Fox, K Allinson, S Sahagun-Krause, H Brown, T |
| author_facet | Fox, K Allinson, S Sahagun-Krause, H Brown, T |
| author_sort | Fox, K |
| collection | OXFORD |
| description | The Vsr mismatch endonuclease recognises the sequence CTWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5'-side of the mispaired thymine. By using base analogues of G and T we have explored the functional groups on the mismatch pair which are recognised by the enzyme. Removal of the thymine 5-methyl group causes a 60% reduction in activity, while removing the 2-amino group of guanine reduces cleavage by 90%. Placing 2-amino-purine or nebularine opposite T generates mis-matches which are cut at a much lower rate (0.1%). When either base is removed, generating a pseudoabasic site (1', 2'-dideoxyribose), the enzyme still produces site-specific cleavage, but at only 1% of the original rate. Although TT and CT mismatches at this position are cleaved at a low rate (approximately 1%), mismatches with other bases (such as GA and AC) and Watson-Crick base pairs are not cleaved by the enzyme. There is also no cleavage when the mismatched T is replaced with difluorotoluene. |
| first_indexed | 2024-03-07T00:03:28Z |
| format | Journal article |
| id | oxford-uuid:76c2dec2-139c-4064-8dc4-afb29e8a8004 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:03:28Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:76c2dec2-139c-4064-8dc4-afb29e8a80042022-03-26T20:18:24ZRecognition of GT mismatches by Vsr mismatch endonuclease.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:76c2dec2-139c-4064-8dc4-afb29e8a8004EnglishSymplectic Elements at Oxford2000Fox, KAllinson, SSahagun-Krause, HBrown, TThe Vsr mismatch endonuclease recognises the sequence CTWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5'-side of the mispaired thymine. By using base analogues of G and T we have explored the functional groups on the mismatch pair which are recognised by the enzyme. Removal of the thymine 5-methyl group causes a 60% reduction in activity, while removing the 2-amino group of guanine reduces cleavage by 90%. Placing 2-amino-purine or nebularine opposite T generates mis-matches which are cut at a much lower rate (0.1%). When either base is removed, generating a pseudoabasic site (1', 2'-dideoxyribose), the enzyme still produces site-specific cleavage, but at only 1% of the original rate. Although TT and CT mismatches at this position are cleaved at a low rate (approximately 1%), mismatches with other bases (such as GA and AC) and Watson-Crick base pairs are not cleaved by the enzyme. There is also no cleavage when the mismatched T is replaced with difluorotoluene. |
| spellingShingle | Fox, K Allinson, S Sahagun-Krause, H Brown, T Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title | Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title_full | Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title_fullStr | Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title_full_unstemmed | Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title_short | Recognition of GT mismatches by Vsr mismatch endonuclease. |
| title_sort | recognition of gt mismatches by vsr mismatch endonuclease |
| work_keys_str_mv | AT foxk recognitionofgtmismatchesbyvsrmismatchendonuclease AT allinsons recognitionofgtmismatchesbyvsrmismatchendonuclease AT sahagunkrauseh recognitionofgtmismatchesbyvsrmismatchendonuclease AT brownt recognitionofgtmismatchesbyvsrmismatchendonuclease |