The effect of tetracyclines on quantitative measures of osteoclast morphology.
We report the effects of the tetracycline analogues 4-dedimethylaminotetracycline (CMT-1) and minocycline on osteoclast spreading and motility. Both agents influenced the morphometric descriptor of cell spread area, rho, producing cellular retraction or an R effect (half-times: 30 and 44 minutes for...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Kluwer Academic Publishers-Plenum Publishers
1993
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| _version_ | 1797076454612140032 |
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| author | Zaidi, M Moonga, B Huang, C Towhidul Alam, A Shankar, V Pazianas, M Eastwood, J Datta, H Rifkin, B |
| author_facet | Zaidi, M Moonga, B Huang, C Towhidul Alam, A Shankar, V Pazianas, M Eastwood, J Datta, H Rifkin, B |
| author_sort | Zaidi, M |
| collection | OXFORD |
| description | We report the effects of the tetracycline analogues 4-dedimethylaminotetracycline (CMT-1) and minocycline on osteoclast spreading and motility. Both agents influenced the morphometric descriptor of cell spread area, rho, producing cellular retraction or an R effect (half-times: 30 and 44 minutes for CMT-1 and minocycline, respectively). At the concentrations employed, the tetracycline-induced R effects were significantly slower than, but were qualitatively similar to, those resulting from Ca2+ "receptor" activation through the application of 15 mM-[Ca2+] (slopes: -1.25, -0.18, and -4.40/minute for 10 mg/l-[CMT-1], 10 mg/l-[minocycline] and 15 mM-[Ca2+], respectively). In contrast, the same tetracycline concentrations did not influence osteoclast margin ruffling activity as described by mu, a motility descriptor known to be influenced by elevations of cellular cyclic AMP. Thus, the tetracyclines exert morphometric effects comparable to changes selectively activated by occupancy of the osteoclast Ca2+ "receptor" which may act through an increase in cytosolic [Ca2+]. |
| first_indexed | 2024-03-07T00:04:01Z |
| format | Journal article |
| id | oxford-uuid:76ef2852-a710-4bc3-9d72-933f9aef7390 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:04:01Z |
| publishDate | 1993 |
| publisher | Kluwer Academic Publishers-Plenum Publishers |
| record_format | dspace |
| spelling | oxford-uuid:76ef2852-a710-4bc3-9d72-933f9aef73902022-03-26T20:19:43ZThe effect of tetracyclines on quantitative measures of osteoclast morphology.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:76ef2852-a710-4bc3-9d72-933f9aef7390EnglishSymplectic Elements at OxfordKluwer Academic Publishers-Plenum Publishers1993Zaidi, MMoonga, BHuang, CTowhidul Alam, AShankar, VPazianas, MEastwood, JDatta, HRifkin, BWe report the effects of the tetracycline analogues 4-dedimethylaminotetracycline (CMT-1) and minocycline on osteoclast spreading and motility. Both agents influenced the morphometric descriptor of cell spread area, rho, producing cellular retraction or an R effect (half-times: 30 and 44 minutes for CMT-1 and minocycline, respectively). At the concentrations employed, the tetracycline-induced R effects were significantly slower than, but were qualitatively similar to, those resulting from Ca2+ "receptor" activation through the application of 15 mM-[Ca2+] (slopes: -1.25, -0.18, and -4.40/minute for 10 mg/l-[CMT-1], 10 mg/l-[minocycline] and 15 mM-[Ca2+], respectively). In contrast, the same tetracycline concentrations did not influence osteoclast margin ruffling activity as described by mu, a motility descriptor known to be influenced by elevations of cellular cyclic AMP. Thus, the tetracyclines exert morphometric effects comparable to changes selectively activated by occupancy of the osteoclast Ca2+ "receptor" which may act through an increase in cytosolic [Ca2+]. |
| spellingShingle | Zaidi, M Moonga, B Huang, C Towhidul Alam, A Shankar, V Pazianas, M Eastwood, J Datta, H Rifkin, B The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title | The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title_full | The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title_fullStr | The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title_full_unstemmed | The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title_short | The effect of tetracyclines on quantitative measures of osteoclast morphology. |
| title_sort | effect of tetracyclines on quantitative measures of osteoclast morphology |
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