exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes
<p>Detecting DNA breaks in defined regions of the genome is critical to advancing our understanding of genome stability maintenance. Here, we present exo-FISH, a protocol to label exposed single-stranded DNA in defined repetitive regions of mammalian genomes by combining <i>in vitro</...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Cell Press
2023
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| _version_ | 1826312039346208768 |
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| author | Saayman, X Graham, E Leung, CWB Esashi, F |
| author_facet | Saayman, X Graham, E Leung, CWB Esashi, F |
| author_sort | Saayman, X |
| collection | OXFORD |
| description | <p>Detecting DNA breaks in defined regions of the genome is critical to advancing our understanding of genome stability maintenance. Here, we present exo-FISH, a protocol to label exposed single-stranded DNA in defined repetitive regions of mammalian genomes by combining <i>in vitro</i> restriction enzyme digestion on fixed cells with fluorescence <i>in situ</i> hybridization (FISH). We describe steps for cell harvesting and fixation, slide treatments, and FISH probe hybridization. We then detail procedures for imaging and analysis.</p>
<p>For complete details on the use and execution of this protocol, please refer to Saayman et al. (2023).<p> |
| first_indexed | 2024-03-07T08:21:42Z |
| format | Journal article |
| id | oxford-uuid:773745eb-c69f-423d-8eed-957232b73af6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:21:42Z |
| publishDate | 2023 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:773745eb-c69f-423d-8eed-957232b73af62024-02-02T11:44:54Zexo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:773745eb-c69f-423d-8eed-957232b73af6EnglishSymplectic ElementsCell Press2023Saayman, XGraham, ELeung, CWBEsashi, F<p>Detecting DNA breaks in defined regions of the genome is critical to advancing our understanding of genome stability maintenance. Here, we present exo-FISH, a protocol to label exposed single-stranded DNA in defined repetitive regions of mammalian genomes by combining <i>in vitro</i> restriction enzyme digestion on fixed cells with fluorescence <i>in situ</i> hybridization (FISH). We describe steps for cell harvesting and fixation, slide treatments, and FISH probe hybridization. We then detail procedures for imaging and analysis.</p> <p>For complete details on the use and execution of this protocol, please refer to Saayman et al. (2023).<p> |
| spellingShingle | Saayman, X Graham, E Leung, CWB Esashi, F exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title | exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title_full | exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title_fullStr | exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title_full_unstemmed | exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title_short | exo-FISH: protocol for detecting DNA breaks in repetitive regions of mammalian genomes |
| title_sort | exo fish protocol for detecting dna breaks in repetitive regions of mammalian genomes |
| work_keys_str_mv | AT saaymanx exofishprotocolfordetectingdnabreaksinrepetitiveregionsofmammaliangenomes AT grahame exofishprotocolfordetectingdnabreaksinrepetitiveregionsofmammaliangenomes AT leungcwb exofishprotocolfordetectingdnabreaksinrepetitiveregionsofmammaliangenomes AT esashif exofishprotocolfordetectingdnabreaksinrepetitiveregionsofmammaliangenomes |