Raman-DIP (Deuterium Isotope Probing) reveals microbial metabolism at the single-cell level

We illustrate that single-cell Raman microspectroscopy, coupled with deuterium isotope probing (Raman-DIP), provides a culture-independent and non-destructive approach to probe metabolic pathways of carbon substrates at the single-cell level. We find a distinguishable CD vibration band at 2070 - 230...

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Bibliographic Details
Main Authors: Xu, J, Zhu, D, Ibrahim, A, Allen, C, Gibson, C, Fowler, P, Song, Y, Huang, W
Format: Journal article
Published: American Chemical Society 2017