Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin.
The mitochondrial matrix contains endogenously biotinylated proteins. These proteins can cause unexpected background signal when biotin-avidin- or biotin-streptavidin-based detection systems are used in immunocytochemistry. Here we show that this reactivity can be deliberately exploited, using a sim...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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